Coding

Part:BBa_K2082107:Design

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-13)


Wild-type polymerase I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 708
    Illegal XhoI site found at 1206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1671


Design Notes

Part obtained from Manel Camps.
Used under control of lac-promoter in temperature-sensitive E. coli strain JS200 on plasmid with pSC101-origin pHSG.


Source

origin: E. coli genome

References